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KMID : 1098420030110040316
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Korean Journal of Medicinal Crop Science
2003 Volume.11 No. 4 p.316 ~ p.320
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An Efficient In vitro Propagation of Zanthoxylum piperitum DC
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Hwang Sung-Jin
Hwang Baik
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Abstract
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A protocol is described for rapid multiplication of Zanthoxylum piperitum DC. (Rutaceae), an important aromatic and medicinal plant, through shoot-tip explant cultures. Murashige and Skoog (MS) medium supplemented with various concentrations of N-6-benzyladenine (BA), N-6-benzylaminopurine (BAP) and thidiazuron(TDZ), in single or in combination with α-naphthaleneacetic acid (NAA), was used to determine the rate of shoot proliferation N-6-benzyladenine (BA) used at 0.5 ㎎/ℓ was the most effective in initiating multiple shoot proliferation at the rate of 23 microshoots per shoot-tip explants after 40 days of culture. Shoot multiplication increased 1.2-fold in each successive subculture. Induction of rooting (98%) was achieved by transferring the shoots to the same basal medium containing 2 ㎎/ℓ indole-3-butyric acid (IBA). Plantlets went through a hardening phase in a controlled growth chamber, prior to in Vivo transfer. These results represented that possible application for the mass production of plantlets through in vitro culture system of Zanthoxylum piperiturn DC.
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